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Mitochondrial DNA-like sequence in the nuclear genome of Saguinus (Callitrichinae, Primates): transfer estimation Genet. Mol. Biol.
Vallinoto,Marcelo; Sena,Leonardo; Sampaio,Iracilda; Schneider,Horacio; Schneider,Maria Paula.
Mitochondrial DNA-like sequences have been found in the nuclei of a variety of organisms. These nuclear pseudogenes can be used to estimate relative evolutionary rates of mitochondrial genes, and can be used as outgroups in phylogenetic analyses. In this study, mitochondrial sequences with pseudogene-like characteristics, including deletions and/or insertions and stop codons, were found in tamarins (Saguinus spp., Callitrichinae, Primates). Phylogenetic analysis allowed estimation of the timing of the migration of these sequences to the nuclear genome, and also permitted inferences on the phylogeny of the genus. The choice of an inadequate outgroup (Aotus infulatus) prevented a good phylogenetic resolution of the subfamily Callitrichinae. The relatively...
Tipo: Info:eu-repo/semantics/article
Ano: 2000 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572000000100006
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Molecular studies of Callithrix pygmaea (Primates, Platyrrhini) based on transferrin intronic and ND1 regions: implications for taxonomy and conservation Genet. Mol. Biol.
Tagliaro,Claudia Helena; Schneider,Maria Paula Cruz; Schneider,Horacio; Sampaio,Iracilda; Stanhope,Michael.
Traditional classifications of Platyrrhini monkeys, based mainly on morphological features, are being contested by recent molecular data. The subfamily Callitrichinae (Platyrrhini, Primates) consists of a diverse group of species, many of them considered endangered. Our analysis of two DNA regions, a mtDNA gene (ND1) and a nuclear gene (intronic regions of the transferrin gene), suggests that Callithrix pygmaea may have sufficient variability to justify the existence of subspecies or even separate species. Phylogenetic dendrograms based on the ND1 region show that this species is more closely related to Amazonian than to Atlantic forest marmosets. These results reopen the discussion about diversity and conservation programs based exclusively on traditional...
Tipo: Info:eu-repo/semantics/article
Ano: 2000 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572000000400006
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Phylogenetic analysis of 16S mitochondrial DNA data in sloths and anteaters Genet. Mol. Biol.
Barros,Maria Claudene; Sampaio,Iracilda; Schneider,Horacio.
We sequenced part of the 16S rRNA mitochondrial gene in 17 extant taxa of Pilosa (sloths and anteaters) and used these sequences along with GenBank sequences of both extant and extinct sloths to perform phylogenetic analysis based on parsimony, maximum-likelihood and Bayesian methods. By increasing the taxa density for anteaters and sloths we were able to clarify some points of the Pilosa phylogenetic tree. Our mitochondrial 16S results show Bradypodidae as a monophyletic and robustly supported clade in all the analysis. However, the Pleistocene fossil Mylodon darwinii does not group significantly to either Bradypodidae or Megalonychidae which indicates that trichotomy best represents the relationship between the families Mylodontidae, Bradypodidae and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Pilosa; Sloths; Anteaters; Phylogeny; Mitochondrial DNA; RDNA16S.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572003000100002
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Genetic differentiation of Macrodon ancylodon (Sciaenidae, Perciformes) populations in Atlantic coastal waters of South America as revealed by mtDNA analysis Genet. Mol. Biol.
Santos,Simôni; Schneider,Horacio; Sampaio,Iracilda.
The king weakfish (pescada-gó in Portuguese - Macrodon ancylodon (Sciaenidae), a demersal (bottom-feeding) species found in South America Atlantic coastal waters from the Gulf of Paria in Venezuela to Baia Blanca in Argentina, is an economically important species because of its abundance and wide acceptance by consumers. Because of its wide distribution this fish may be subject to geographic isolation and this may have resulted in distinct populations along its coastal range. Considering that this species represents an important economic resource, confirmation of whether M. ancylodon is a single species or there are different genetic stocks spread over its wide distribution would be an important contribution to conservation policies and population...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Macrodon ancylodon; Sciaenidae; Perciformes; 16S rRNA; Cytochrome b; Genetic differentiation.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572003000200008
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The prion protein and New World primate phylogeny Genet. Mol. Biol.
Schneider,Igor; Schneider,Horacio; Schneider,Maria Paula; Silva,Artur.
The PrP C prion protein contains 250 amino acids with some variation among species and is expressed in several cell types. PrP C is converted to PrP Sc by a post-translational process in which it acquires amino acid sequences of three-dimensional conformation of beta-sheets. Variations in the prion protein gene were observed among 16 genera of New World primates (Platyrrhini), and resulted in amino acid substitutions when compared with the human sequence. Seven substitutions not yet described in the literature were found: W -> R at position 31 in Cebuella, T -> A at position 95 in Cacajao and Chiropotes, N-> S at position 100 in Brachyteles, L -> Q at position 130 in Leontopithecus (in the sequence responsible for generating the beta-sheet 1),...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Prion protein; New World monkeys; Molecular phylogeny.
Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572004000400007
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Sciaenidae fish of the Caeté River estuary, Northern Brazil: mitochondrial DNA suggests explosive radiation for the Western Atlantic assemblage Genet. Mol. Biol.
Vinson,Christina; Gomes,Grazielle; Schneider,Horacio; Sampaio,Iracilda.
Sciaenids are fish which are normally abundant in tropical estuaries of the western Atlantic. Studies on the Caeté river estuary in the northern Brazilian state of Pará have revealed that in this area Sciaenidae is the dominant family, comprising almost 50% of all teleosts sampled. In this paper we present the results of the first phylogenetic study on South American estuarine sciaenids, during which we obtained mitochondrial gene 16S sequences from 15 species belonging to eight genera occurring in the Caeté estuary. Intergeneric nucleotide divergences varied from 5 to 15%, Lonchurus and Menticirrhus being the most divergent lineages. Nucleotide divergences were quite variable amongst species of the same genus, ranging from 1.2% (Stellifer microps x...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Sciaenidae; Perciformes; Mitochondrial DNA; RRNA 16S; Caeté River estuary.
Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572004000200008
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Molecular phylogeny of the genus Saguinus (Platyrrhini, Primates) based on the ND1 mitochondrial gene and implications for conservation Genet. Mol. Biol.
Tagliaro,Claudia Helena; Schneider,Horacio; Sampaio,Iracilda; Schneider,Maria Paula Cruz; Vallinoto,Marcelo; Stanhope,Michael.
The systematics of the subfamily Callitrichinae (Platyrrhini, Primates), a group of small monkeys from South America and Panama, remains an area of considerable discussion despite many investigations, there being continuing controversy over subgeneric taxonomic classifications based on morphological characters. The purpose of our research was to help elucidate the phylogenetic relationships within the monkey genus Saguinus (Callitrichinae) using a molecular approach to discover whether or not the two different sections containing hairy-faced and bare-faced species are monophyletic, whether Saguinus midas midas and Saguinus bicolor are more closely related than are S. midas midas and Saguinus midas niger, and if Saguinus fuscicollis melanoleucus and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Saguinus; Callitrichinae; ND1; Phylogeny; MtDNA; Conservation.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000100009
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Taxonomic implications of molecular studies on Northern Brazilian Teredinidae (Mollusca: Bivalvia) specimens Genet. Mol. Biol.
Santos,Sonia Maria Lima; Tagliaro,Claudia Helena; Beasley,Colin Robert; Schneider,Horacio; Sampaio,Iracilda; Santos Filho,Carlos; Müller,Ana Cláudia de Paula.
The current taxonomy of the Teredinidae (shipworms) is wholly based on morphology and up to now no molecular studies of the phylogeny of this group have been published. In the present study the relationships between four genera of the subfamilies Teredininae and Bankiinae were established and the efficiency of the 16S rRNA gene in characterizing four Teredinidae species was tested. Phylogenetic trees support the grouping of Bankia fimbriatula with Nausitora fusticula and of Neoteredo reynei with Psiloteredo healdi, but the genetic distances do not justify the classification of these species into two distinct subfamilies. The results show that B. fimbriatula, N. reynei and P. healdi specimens from the coast of the Brazilian state of Pará have five distinct...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Teredinidae; RRNA16S; Phylogeny; MtDNA.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000100031
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Tocantins river as an effective barrier to gene flow in Saguinus niger populations Genet. Mol. Biol.
Vallinoto,Marcelo; Araripe,Juliana; Rego,Péricles S. do; Tagliaro,Claudia H.; Sampaio,Iracilda; Schneider,Horacio.
The Saguinus represent the basal genus of the Callitrichinae subfamily. Traditionally this genus is divided into three groups: Hairy, Mottled and Bare-face, however, molecular data failed to validate these groups as monophyletic units, as well as raised some subspecies to the species status. This is the case of the former subspecies Saguinus midas midas and S. midas niger, which are now considered as different species. In the present study, we sequenced a portion of the D-loop mtDNA region in populations from the East bank of the Xingu and from both banks of the Tocantins river, in order to test the effectiveness of large rivers as barriers to the gene flow in Saguinus. According to our results, the populations from the East and West banks of the Tocantins...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Primates; Saguinus niger; D-loop; MtDNA; Tocantins river.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000200005
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Molecular discrimination of pouched four-eyed opossums from the Mamirauá Reserve in the Brazilian Amazon Genet. Mol. Biol.
Nunes,Cláudia; Ayres,Jose Marcio; Sampaio,Iracilda; Schneider,Horacio.
Previous cytochrome B (CytB) mtDNA studies have suggested four species for the opossum genus Philander (four-eyed opossums), three (P. mcilhennyi, P. andersoni and P. opossum) from the Amazon and one (P. frenata) from the Brazilian Atlantic forest. During a faunal survey nine specimens of Philander sp. and four of Didelphis marsupialis were collected in the Mamirauá Sustainable Reserve, Amazonas State, Brazil. Preliminary analyses based on morphology and geographical distributions were not conclusive, suggesting that Philander specimens could belong to either P. andersoni or P. opossum. In order to elucidate the relationship of this taxon to the remaining Amazonian taxa, seven Philander and two Didelphis specimens animals were sequenced for the cytB mtDNA...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cytochrome B; Mamirauá; Molecular phylogeny; MtDNA; Philander; Pouched four-eyed opossum.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000200015
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Novel 12S mtDNA findings in sloths (Pilosa, Folivora) and anteaters (Pilosa, Vermilingua) suggest a true case of long branch attraction Genet. Mol. Biol.
Barros,Maria Claudene; Sampaio,Iracilda; Schneider,Horacio.
We sequenced 12S RNA mtDNA for the majority of the extant species of sloths and anteaters and compared our results with previous data obtained by our group using 16S RNA mtDNA in the same specimens and to GenBank sequences of the extinct giant sloth Mylodon. Our results suggest that pigmy-anteaters may be a case of the long-branch attraction phenomenon and also show the large genetic difference between the Amazonian and Atlantic forest three-toed sloths, contrasting with the small differences observed between the two non-Atlantic forest forms of sloths. These results have important implications for the taxonomy of sloths and anteaters and strongly suggest the placement of pigmy anteaters in their own family (Cyclopidae) and raising the taxonomic status of...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Anteaters; Molecular evolution; Mylodont; Sloths; Xenarthra.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000400029
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Low levels of genetic diversity depicted from mitochondrial DNA sequences in a heavily exploited marine fish (Cynoscion acoupa, Sciaenidae) from the Northern coast of Brazil Genet. Mol. Biol.
Rodrigues,Rosa; Schneider,Horacio; Santos,Simôni; Vallinoto,Marcelo; Sain-Paul,Ulrich; Sampaio,Iracilda.
The acoupa weakfish (Cynoscion acoupa - Sciaenidae) is a marine species of croaker with estuarine-dependent behavior, found in the western Atlantic from Panama to Argentina. It is one of the most exploited food fish on the northern coast of Brazil. In this study, DNA sequences were determined from the entire control region (D-loop) of the mitochondrial genome of 297 individuals collected during seven different months between December 2003 and August 2005 on the northern coast of Brazil (Amapá and Pará). Genetic variability expressed by haplotype (h = 0,892) and nucleotide (pi = 0,003) diversities were low compared to other heavily exploited marine fish species from the western Atlantic and eastern Asia. AMOVA depicted a lack of genetic structuring among...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cynoscion acoupa; Acoupa weakfish; Sciaenidae; Low genetic diversity; Mitochondrial DNA; D-loop.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000300015
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Can Lutjanus purpureus (South red snapper) be "legally" considered a red snapper (Lutjanus campechanus)? Genet. Mol. Biol.
Gomes,Grazielle; Schneider,Horacio; Vallinoto,Marcelo; Santos,Simoni; Orti,Guillermo; Sampaio,Iracilda.
Red snappers (Lutjanus purpureus in Brazil and Lutjanus campechanus in USA and Gulf of Mexico) are both under clear effect of overfishing. Because of their high morphological similarity it has already been suggested that they could possibly be considered as a single species. To investigate the degree of similarity and the genetic structure of red snapper populations we constructed a common dataset of partial D-loop mtDNA sequences of L. purpureus from Brazil (Amapá, Pará and Maranhão) and L. campechanus from the Atlantic coast of the USA (Florida, Louisiana and Mississippi). Phylogenetic and population genetic analyses surprisingly depicted high similarity between L. campechanus and L. purpureus, compatible with the hypothesis of a single species of red...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Red snapper; Lutjanus purpureus; Lutjanus campechanus; Population genetic structure; Mitochondrial D-loop.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000200035
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Genetic characterisation of populations of the critically endangered Goliath grouper ( Epinephelus itajara, Serranidae) from the Northern Brazilian coast through analyses of mtDNA Genet. Mol. Biol.
Silva-Oliveira,Gláucia C.; Rêgo,Péricles S. do; Schneider,Horacio; Sampaio,Iracilda; Vallinoto,Marcelo.
The Goliath grouper ( Epinephelus itajara ) is one of the most endangered species of fish of the subfamily Epinephelinae. Slow to develop and mature, and dependent on mangrove habitats for breeding, the species also suffers intense harvesting, which has reduced drastically in numbers in many areas. To contribute to the understanding of the characteristics of E. itajara populations, we conducted a molecular genetics study of the species, focusing on populations from the Northern Brazilian coast. The mtDNA control region (D-loop) of 116 individuals from five localities (Bragança, Ajuruteua, Parnaíba, Fortaleza and Natal) was analysed, and a sequence of 499 base pairs identified. Analyses of the sequences indicated that genetic variability was generally...
Palavras-chave: Goliath grouper; Epinephelus itajara; Control region; MtDNA; Population genetics.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000500029
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Genetic characterization of native and introduced populations of the neotropical cichlid genus Cichla in Brazil Genet. Mol. Biol.
Carvalho,Daniel Cardoso de; Oliveira,Denise Aparecida Andrade de; Santos,José Enemir dos; Teske,Peter; Beheregaray,Luciano B.; Schneider,Horacio; Sampaio,Iracilda.
A molecular phylogenetic analysis based on mitochondrial 16S ribosomal DNA and Control Region sequences from native and introduced populations was undertaken, in order to characterize the introduction of Cichla (peacock bass or tucunaré) species in Brazil. Mitochondrial DNA haplotypes found in introduced fish from Minas Gerais state (southeastern Brazil) clustered only with those from native species of the Tocantins River (Cichla piquiti and C. kelberi), thereby suggesting a single or, at most, few translocation acts in this area, even though with fish from the same source-population. Our study contributes to an understanding of the introduction of Cichla in regions of Brazil outside the Amazon basin, and adds phylogenetic data to the recently describe...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Peacock bass or tucunaré; MtDNA; Invasive populations; Amazon basin; Minas Gerais.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000300025
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Identification and phylogenetic inferences on stocks of sharks affected by the fishing industry off the Northern coast of Brazil Genet. Mol. Biol.
Rodrigues-Filho,Luis Fernando da Silva; Rocha,Tainá Carreira da; Rêgo,Péricles Sena do; Schneider,Horacio; Sampaio,Iracilda; Vallinoto,Marcelo.
The ongoing decline in abundance and diversity of shark stocks, primarily due to uncontrolled fishery exploitation, is a worldwide problem. An additional problem for the development of conservation and management programmes is the identification of species diversity within a given area, given the morphological similarities among shark species, and the typical disembarkation of processed carcasses which are almost impossible to differentiate. The main aim of the present study was to identify those shark species being exploited off northern Brazil, by using the 12S-16S molecular marker. For this, DNA sequences were obtained from 122 specimens collected on the docks and the fish market in Bragança, in the Brazilian state of Pará. We identified at least 11...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Sharks; Molecular identification; Phylogenetic; MtDNA; Conservation.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000200031
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Molecular phylogenies, chromosomes and dispersion in Brazilian akodontines (Rodentia, Sigmodontinae) Iheringia, Sér. Zool.
Barros,Maria Claudene; Sampaio,Iracilda; Schneider,Horacio; Langguth,Alfredo.
A new molecular phylogeny for akodontine rodents from Brazil was proposed. The phylogenetic tree was enriched with the area of occurrence and with information on the karyotype of the samples. Based on this enriched tree, and with a described methodology, hypotheses were proposed on the karyotype and area of occurrence of the ancestors of each Clade. Thus it was possible to discuss hypotheses on chromosome evolution of the group, and on dispersion events from the "area of original differentiation" of akodontines in the Andes. Chromosome evolution started with high diploid numbers (2n=52) and showed a tendency to reduction (until 2n=14 in more recent clades). Independent side-branches of the tree showed 2n reduction and in one case the 2n increased. At least...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Brazilian akodontines; Chromosome evolution; Biogeography; Molecular phylogeny.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0073-47212009000400006
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Molecular identification, phylogeny and geographic distribution of Brazilian mangrove oysters (Crassostrea) Genet. Mol. Biol.
Melo,Aline Grasielle Costa de; Varela,Eduardo Sousa; Beasley,Colin Robert; Schneider,Horacio; Sampaio,Iracilda; Gaffney,Patrick Michael; Reece,Kimberly S.; Tagliaro,Claudia Helena.
Oysters (Ostreidae) manifest a high degree of phenotypic plasticity, whereby morphology is of limited value for species identification and taxonomy. By using molecular data, the aim was to genetically characterize the species of Crassostrea occurring along the Brazilian coast, and phylogenetically relate these to other Crassostrea from different parts of the world. Sequencing of the partial cytochrome oxidase c subunit I gene (COI), revealed a total of three species of Crassostrea at 16 locations along the Brazilian coast. C. gasar was found from Curuçá (Pará state) to Santos (São Paulo state), and C. rhizophorae from Fortim (Ceará state) to Florianópolis (Santa Catarina state), although small individuals of the latter species were also found at Ajuruteua...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Genetic identification; Oysters; Biogeography; COI gene; Ostreidae.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000300030
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The history of the introduction of the giant river prawn, Macrobrachium cf. rosenbergii (Decapoda, Palaemonidae), in Brazil: new insights from molecular data Genet. Mol. Biol.
Iketani,Gabriel; Pimentel,Luciana; Silva-Oliveira,Glaúcia; Maciel,Cristiana; Valenti,Wagner; Schneider,Horacio; Sampaio,Iracilda.
The giant river prawn, Macrobrachium cf. rosenbergii, is one of the most cultivated freshwater prawns in the world and has been introduced into more than 40 countries. In some countries, this prawn is considered an invasive species that requires close monitoring. Recent changes in the taxonomy of this species (separation of M. rosenbergii and M. dacqueti) require a re-evaluation of introduced taxa. In this work, molecular analyses were used to determine which of these two species was introduced into Brazil and to establish the geographic origin of the introduced populations that have invaded Amazonian coastal waters. The species introduced into Brazil was M. dacqueti through two introduction events involving prawns originating from Vietnam and either...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bioinvasion; Exotic species; Giant river prawn; Macrobrachium dacqueti; COI; 16S rRNA; Amazonia.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000100025
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Occurrence of the Indo-Pacific freshwater prawn Macrobrachium equidens Dana 1852 (Decapoda, Palaemonidae) on the coast of Brazilian Amazonia, with notes on its reproductive biology Anais da ABC (AABC)
Maciel,Cristiana R; Quadros,Manoel L; Abrunhosa,Fernando; Bastos,Sandra; Schneider,Horacio; Sampaio,Iracilda.
The freshwater prawn Macrobrachium equidens, which is native species of the Indo-Pacific Region, was recorded for the first time on the Amazon coast of Brazil. This species was found to inhabit the same environment as two native Macrobrachium species, M. amazonicum and M. acanthurus, and is morphologically very similar to the latter. The identification of the species was confirmed by the genetic analysis of sequences of the mitochondrial Cytochrome Oxidase (COI) gene. A detailed description of the morphological features and reproductive biology of M. equidens in this new environment is presented.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Crustacean; Caeté Estuary; Invasive species; Mitochondrial COI gene; Reproductive biology.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652011000200013
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